10-180kDa Prestained Protein Ladder
Cat. No. PL0500 Size: 500 μl
Store at -20°C for 24 months
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively.
• 2~3 μl per well for general Western transfering.
• Apply more for thicker (>1.5 mm) or larger gel.
The 10-180kDa Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-180kDa Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes(PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol).
5 μl of 10-180kDa Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each
For research use only
The purpose of Slider Kit is to gather common slideshow-like jQuery functionalities (such as news sliders, photos galleries/sliders, carousels, tabs menus)
When tracking mouse movement, you usually need to know the actual position of the mouse pointer. The event object that is passed to the handler contains some information about the mouse coordinates.