FB Plasmid Midiprep Kit (20 rxn)
Sample: up to 100 ml bacterial cells
Yield: up to 250 μg of plasmid
The FB Plasmid Midiprep Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolation of plasmid DNA from cultured bacterial cells. The FB Plasmid Midiprep Kit is based on alkaline lysis of bacterial cells followed by binding of DNA onto the glass fiber matrix of the spin column in the presence of high salt (1, 2).Phenol extraction and ethanol precipitation are not required, and high-quality plasmid DNA is eluted in a small volume of Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). Plasmid DNA purified with FB Plasmid Midiprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, Sequencing, library screening, in vitro translation, transfection of robust cells,ligation and transformation. The entire procedure can be completed within 30-40 minutes.
For research use only
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