Key FeaturesThis plasmid kit was designed for plasmid and cosmid DNA purification from 1-7 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) in included to prevent common handling errors while ensuring efficient cell lysis, neutralization and maximum plasmid product yield. uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation are not required and the entire procedure can be completed within 15 minutes.
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