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Viogene VioTotal Plant RNA Miniprep Extraction System

Model No.
PVTR1001、PVTR1002
Made in
Taiwan
Supplier
VIOGENE-BIOTEK CORPORATION
Request for Quotation

Key Features

For Plant Samples

  • Introduction

Viogene® RNA Extraction Systems provides a simple silica-gel-membrane spin-column and economical method to purify total RNA from various samples: Traditional Viogene RNA Extraction System extraction total RNA from cultured cells, tissues, bacteria, yeast and plants; miTotal RNA Extraction System for total RNA extraction emphasizing on microRNA and siRNA yield and purification, suitable for animal cells from culture, tissues, whole blood, plasma, serum, biological fluids containing RNA virus, etc.. ; and VioTotal Plant RNA Extraction System provides a fast method to purify total RNA from plant material and plant cells.

 

·         Principle

Viogene® VioTotalTM Plant RNA Extraction Miniprep System provides a fast method to purify total RNA from 100 mg plant material or 1 x 107 plant cells. A simple spin-column based method can isolate large RNAs, siRNAs, and microRNAs without the time-consuming procedure of phenol/chloroform extraction and ethanol precipitation.

  • Features and benefits
    • Enhanced small and microRNA yield 
    • Purification process requires only 30 minutes
    • Spin column fit by centrifugation
    • No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
    • Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material

 

RNA yields from Viral Nucleic Acids Extraction System

 

Sample Size

Elution Volume

Yield

1x104 – 5x106 plant cells, 50mg plant material

30~50 μl

 25-50 µg

 

  • Downstream Application

RNA purified by Viogene VioTotal Plant RNA Extraction System generates high quality of A260/A280 ratio (1.8~2.1), and is ideal for all applications including:

 

    • Northern, dot and slot blotting
    • RT-PCR / Quantitative real-time PCR
    • Poly A+ RNA selection
    • cDNA Synthesis/ Primer extension
    • Array analysis
    • Transcription profiling
    • In vitro translation

 

Ordering Information

Product

Package

Contents

Cat. No

Viogene VioTotal Plant RNA Mini Extraction System

50 preps

50 Plant Total RNA Mini Column,
50 Collection Tubes, Buffers, Protocol,
RNase-free ddH2O

PVTR 1001

250 preps

250 Plant Total RNA Mini Column,
250 Collection Tubes, Buffers, Protocol,
RNase-free ddH2O

PVTR 1002

 

Product Certification


Last Update : 2012-12-13
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